ABSTRACT
Staphylococcus aureus is one of the major pathogen responsible for skin infection, urinary tract infection (UTI) and endocarditis in human. The study was performed to determine the prevalence of multidrug resistant S. aureus in human clinical sample and to evaluate their sensitivity to Allamanda cathartica L. leaf extract. A total of 12 isolates were identified belongs to S. aureus by perform-ing several physiological and biochemical tests. The isolates exhibited highest resistant (75%) to streptomycin and lowest (33.33%) against co-trimoxazole followed by disc diffusion assay of eight antibiotics tested. The other four antibiotics such as azithromycin, chloramphenicol, gentamycin and erythromycin exhibited 50 to 66.67% resistant to present isolates. Here we found that 75% of S. aureus isolates were multidrug-resistant (MDR). The crude leaf extract of A. cathartica L. found to possess antibacterial properties at the rate of 83.33% against S. aureus isolates with 12-22 mm zone of inhibition. Results of TLC states that Benzene : Ethyl acetate (1:1) solvent system was more effective for initial separation of compound from crude leaf extract resulted three distinct bands with different Rf values ranging from 0.53 to 0.89. The result of this study refers that A. cathartica L. leaf extract would be useful to develop effective drugs that would reduce the higher prevalence of multidrug resistance S. aureus causing clinical infection in human.
ABSTRACT
Staphylococcus aureus is one of the major pathogen responsible for skin infection, urinary tract infection (UTI) and endocarditis in human. The study was performed to determine the prevalence of multidrug resistant S. aureus in human clinical sample and to evaluate their sensitivity to Allamanda cathartica L. leaf extract. A total of 12 isolates were identified belongs to S. aureus by perform-ing several physiological and biochemical tests. The isolates exhibited highest resistant (75%) to streptomycin and lowest (33.33%) against co-trimoxazole followed by disc diffusion assay of eight antibiotics tested. The other four antibiotics such as azithromycin, chloramphenicol, gentamycin and erythromycin exhibited 50 to 66.67% resistant to present isolates. Here we found that 75% of S. aureus isolates were multidrug-resistant (MDR). The crude leaf extract of A. cathartica L. found to possess antibacterial properties at the rate of 83.33% against S. aureus isolates with 12-22 mm zone of inhibition. Results of TLC states that Benzene : Ethyl acetate (1:1) solvent system was more effective for initial separation of compound from crude leaf extract resulted three distinct bands with different Rf values ranging from 0.53 to 0.89. The result of this study refers that A. cathartica L. leaf extract would be useful to develop effective drugs that would reduce the higher prevalence of multidrug resistance S. aureus causing clinical infection in human.
ABSTRACT
The studies were carried out to evaluate antibacterial activity of 35 aqueous herbal extracts against a total of 20 clinical Klebshiella sp. isolates. The maximum antibacterial activity was found as 90% in crude extracts of Syzygium aromaticum (leaf) and Citrus limon L. (fruit) followed by 85% in Spondias pinnata (leaf). Sensitivity of these isolates was also evaluated for eight commercial antibiotic discs following disc diffusion assay where most of the isolates found to develop resistance against multiple commercial antibiotics. 85% of isolates exhibited resistant to chloramphenicol and erythromycin and 80% were resiatant to sulfamethoxazole and cephradine. The isolates showed their resistance between 55-60 % to the other four antibiotic discs, viz; gentamycin, streptomycin, ciprofloxacin and azithromycin. Among 35 herbal extracts tested, 19 herbal extrats were found to possess antimicrobial activity in all multi-drug resistant isolates. Therefore these herbal extracts could be used in future direction as alternative therapeutic agents for the treatment of human diseases caused by Klebsiella sp.
ABSTRACT
The studies were carried out to evaluate antibacterial activity of 35 aqueous herbal extracts against a total of 20 clinical Klebshiella sp. isolates. The maximum antibacterial activity was found as 90% in crude extracts of Syzygium aromaticum (leaf) and Citrus limon L. (fruit) followed by 85% in Spondias pinnata (leaf). Sensitivity of these isolates was also evaluated for eight commercial antibiotic discs following disc diffusion assay where most of the isolates found to develop resistance against multiple commercial antibiotics. 85% of isolates exhibited resistant to chloramphenicol and erythromycin and 80% were resiatant to sulfamethoxazole and cephradine. The isolates showed their resistance between 55-60 % to the other four antibiotic discs, viz; gentamycin, streptomycin, ciprofloxacin and azithromycin. Among 35 herbal extracts tested, 19 herbal extrats were found to possess antimicrobial activity in all multi-drug resistant isolates. Therefore these herbal extracts could be used in future direction as alternative therapeutic agents for the treatment of human diseases caused by Klebsiella sp